• OP Labs formerly Oxford Peptides
• Manufactured to defined specification
• Store in a cool dark place
• Sold for research purposes only
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Download COA here: Vitamin_B12_COA.pdf
Please note: if you have a different Batch ID, please contact us for the latest COA.

Vitamin B-12

Synonyms / Designations: Vitamin B-12, Cobalamin, Cyanocobalamin (commonly used form), Hydroxocobalamin, Methylcobalamin.
CAS Number: 68-19-9 (Cyanocobalamin reference form)
Molecular Formula: C63H88CoN14O14P
Molecular Weight:~1355.38 g/mol
Compound Classification: Naturally occurring cobalt-containing corrinoid vitamin
Purity: Manufactured to defined specification (HPLC / spectroscopic methods, typical)
Appearance: Red to dark red crystalline or lyophilised powder
Pack Size: Variable (research-grade)
Storage: Desiccated, protected from light, stored at –20 °C
Solubility: Soluble in water; slightly soluble in ethanol; insoluble in non-polar solvents

Description & Mechanism

Vitamin B-12 is a water-soluble, cobalt-containing vitamin essential for fundamental biochemical processes in cellular metabolism. It functions as a cofactor for key enzymatic reactions involved in one-carbon metabolism, nucleic acid synthesis, and mitochondrial energy regulation.

In biochemical and cellular research systems, Vitamin B-12–dependent enzymes such as methionine synthase and methylmalonyl-CoA mutase are studied for their roles in DNA synthesis, amino acid metabolism, and fatty acid processing. Experimental models utilise Vitamin B-12 to investigate cofactor-dependent enzymatic activity, intracellular transport mechanisms, and nutrient–enzyme interactions under controlled conditions.

Applications in Research

• Investigation of one-carbon metabolism and methylation pathways
• Study of cofactor-dependent enzymatic reactions
• Cellular metabolism and mitochondrial function research
• Nutrient transport and vitamin uptake studies
• Reference compound in biochemical and nutritional research

Handling, Reconstitution & Stability

• Handle using standard laboratory precautions
• Reconstitute in sterile water or appropriate aqueous buffer
• Protect solutions from light exposure
• Aliquot reconstituted solutions to avoid repeated freeze–thaw cycles
• Store reconstituted solutions at 2–8 °C for short-term use or –20 °C for long-term storage

Specifications Summary

Purity: Manufactured to defined specification
Appearance: Red to dark red liquid
Molecular Weight: ~1355.38 g/mol
Compound Type: Vitamin
Solubility: Water soluble
Storage: –20 °C, desiccated, dark

Precautions & Notes

• Stability may be affected by light, heat, and extreme pH
• Experimental outcomes depend on concentration, model system, and formulation
• Appropriate controls are recommended for enzymatic and cellular assays
• Intended strictly for laboratory research use; not for human or veterinary application

References

Banerjee R., Ragsdale S.W. The many faces of vitamin B12: catalysis by cobalamin-dependent enzymes. Annual Review of Biochemistry, 2003.
https://www.annualreviews.org/doi/10.1146/annurev.biochem.72.121801.161828

O’Leary F., Samman S. Vitamin B12 in health and disease. Nutrients, 2010.
https://www.mdpi.com/2072-6643/2/3/299

Froese D.S., Gravel R.A. Genetics of intracellular vitamin B12 metabolism. Molecular Genetics and Metabolism, 2010.
https://pubmed.ncbi.nlm.nih.gov/21114891/

Keywords: Vitamin B-12, Cobalamin, Cyanocobalamin, Methylcobalamin, One-Carbon Metabolism, Cofactor Enzymes, Cellular Metabolism